Hiragana.co.kr ȸ���: ��������. ����Ư���� ���� �μ� 535-329 ����ڹ�ȣ:110-02-65323. ����Ǹž� �Ű���ȣ: ���� 12316ȣ ��������å�� �� ��ǥ�� : �����. ��ȭ: 0505-780-7070 �ѽ�: 0505-780-7071 �̸���:lee�����hiragana.co.kr *���������۹� ������ �����Ʈ���� �����ϴ� ������������ ���۱Ǻ�ȣ�� ������ �ҹ������� ���մϴ�. 지난 7월 일본에 다녀왔습죠..ㅎㅎ 그러자면 일본어의 가장 기본이되는 그래서!!! 고딩시절 일본어를 제2외국어로 배우기는 했지만 ○ 벼 종자수출 대상국가 핵심 유전자원 및 leading variety들과 다수성, 병해충 저항성 및 향미 등 주요 자원들rks 교잡으로 813 조합의 F1을 육성하였음. 캄보디아의 주요 품종 Chulsa, Senpidao, 베트남 주요 품종 AS996-9, OM4900, OM8108, 미얀마 주요 품종 Sin Thwe Lat, Sin Thu Kha, Thee Thup Yip, 인도네시아 주요 품종 Ciherang and Inpari 19 등을 활용하였으며, 향미벼 유전자원 다양화를 위해 인도 Basmati 향미벼 Basmati 370, DP, Pusa1121, Sharbati, 태국 자스민 품종 Jasmine85 등을 교배에 활용하였다. ○ 교잡된 813개 F1들 중 607 조합 F1은 캄보디아 육종기지에서 육성하여 출수기, 초형, 이삭모양, 립형 등을 평가하여 유망한 106 조합 F1을 선발하여 F2 세대로 진전하였다. 2016년 우기에 선발된 31 조합 F1은 2017년 건기에 F2 세대로 육성될 예정이다. F2 세대로 진전된 106 조합들은 집단의 출수기, 초형, 수량 구성요소 특성 등을 평가하여 62 조합에서 2,560 개체를 선발하여 F3 세대로 진전하였다. ○ 2014∼2016년의 6 작기 동안 작기별 508∼1,680 계통, 전체 6,256 계통의 F3 이후 세대를 육성하였으며, 이들도 출수기, 초형, 수량 구성요소 특성 등을 평가하여 유망한 계통에서 우량한 3개체씩을 선발하였다. 기후변화에 대응하기 위하여 내염성 HZB-SAL6–SAL3, HHZ8-SAL14-SAL3-Y2, HHZ8-SAL12-Y2-DT1, 내건성 IR79643-39-2-2-3, IR78581-12-3-2-2, 내열성 Dular, N22, 흰잎마름병 저항성 Xa4, xa5 보유 Angke, 도열병 저항성 유전자 Pi5 보유 태백, 바이러스 저항성 TW16 등을 교잡한 조합에서 육성된 유망 계통 선발에 유의하였다. ○ 육성된 고세대 계통에 대해 수량성을 평가한 결과 ○ 병해충 검정가능 국가 선정 ○ 병해충 및 내재해성 유전자/분자 마커 개발 및 선발체계 구축 ○ 수출 대상국 병해충 및 내재해성 유전자 연관 분자표지 개발 ○ 병해충저항성 마커 검정 및 우수 계통 선발 ○ 베트남 쿠롱델타벼연구소(CLRRI)에서 통일형 및 내열성 계통에 대한 적응성 평가시험에서 시험계통들의 생육기간은 104~109일로 비교품종 OM7347 및 OM6162의 생육기간 106일 및 108일과 비슷하였고, 수당립수는 104~162개, 등숙율 70.6~88.3%, 정조 천립중 21.9~27.9g이었고, 수량은 YR29200-37-3-2 계통 외에 7.2∼8.6 ton/ha 이었음. 이들 계통들은 외관이 다소 불투명하고 심복백이 1/2 정도였고, 금후 6.5mm 이상의 장립형으로 쌀 외관이 투명하고 심복백이 없는 상품성이 우수한 고품질 품종육성이 필요함. ○ 미얀마 Hlegu 지역에서 11 계통의 통일형 및 내열성 계통들에 대한 적응성 검정시험에서 생육기간은 100∼108일의 중생군, 수당립수는 112∼158개, 등숙율은 85.2∼95.9% 였고, 벼 수량은 3∼5.75 ton/ha 범위에 4.35 ton/ha이였다. WC625-1-1-3-1-1-1, KR689-B-5-1-3 및 and KR528-b-b-95-2 계통들의 수량은 IR66의 80~92% 수준인 5∼5.75 ton/ha이였음. ○ 베트남 남부 도열병 밭못자리 저항성 검정에서 KR49-1-1이 이병성 반응을 보였고, AS996-9 등 12 계통은 중도 저항성을, 나머지 계통들은 중강∼강의 저항성 반응을 보였고, 베트남 북부 검정에서는 태백(Pi5), IR78581-12-3-2-2, Angke, Conde 등의 핵심 유전자원들과 KR106-48-5, KR57-5-2 등 16 계통들이 중강 저항성 반응을 보였다. 흰잎마름병 검정 결과, IR78581-12-3-2-2, OM8108, Angke (Xa4, xa5), Conde (Xa4, Xa7), Sin Thu Kha 등 5개 핵심 유전자원과 KR54-28-1 등 13 계통이 중강∼강 저항성 반응을 보였음. 저항성을 보인 육성 계통들은 베트남 외 수출 대상국 미얀마, 캄보디아에서도 안정된 저항성을 보일 것으로 추정된다. 바이러스 검정 결과, 태백, IR78581-12-3-2-2, OM8108 등 유전자원과 44개 육성계통들 중 34계통에서 tungro, yellowing, ragged stunt, grassy stunt 바이러스 증상이 나타났음 〈 제2세부과제 : 지중해 연안지역 적응 조숙 중단립형 벼품종 개발 〉 ○ 국내 육성품종의 현지 적응성검정을 위하여 루마니아 브러일라 농업연구소에 육종기지를 구축하고 터키농업연구소, 우즈베키스탄 농림생산성본부와 업무협약을 체결하여 지역적응성 검정을 할 수 있는 공동연구 체계를 구축하였음 ○ 국내에서 육성된 운광벼 등 23품종의 현지 적응성 검정을 2014~2016년간 실시하였고 루마니아에서는 둔내벼, 터키에서는 운광벼와 삼강벼가 우수한 것으로 분석되었으며 둔내벼는 상업화 기반 조성을 위하여 루마니아에 국가목록등재를 신청하였음 ○ 유럽지역 적응 수출용 신규 우량품종 개발을 위하여 유럽 및 국내 자원을 이용하여 246조합의 인공교배를 실시하였으며 F1 ~ F4 및 A1세대를 육성하여 190조합 3,299계통의 유망계통을 선발하였음 ○ 9조합 1,266개체의 수별집단을 양성하였고 9계통의 생산력 검정 예비 계통을 선발하였으며 예비선발계통의 수량성은 운광벼 대비 101~127% 증수되는 계통이었음 ○ 유럽 및 지중해지역 벼 수급현황 및 시장분석을 하였으며 쌀소비 형태 및 소비량, 국가별 육종목표 등을 분석하였음 〈 제3세부과제 : 수출용 벼 내재해성 유전자 분자표지 이용 및 생물검정 체계 구축 〉 ○ 병해충 저항성 검정 성적과 분자표지와의 연관분석 ○ 유망 교배모본 및 후대계통 선발 ○ 기타 연구결과 (출처 : 요약문 p.4) Abstract Sub-project 1: Development of high yield variety of medium and long grain type adaptable to tropical Asia for exporting rice seed... Sub-project 1: Development of high yield variety of medium and long grain type adaptable to tropical Asia for exporting rice seed We selected core germplasm including leading varieties of target countries for exporting rice seed and crossed 813 F1 between core germplasm and promising varieties/lines of high yield potential, resistance to biotic and abiotic stresses, aromatic rice, and so on. The F1s were crossed with Chulsa, Senpidao for Cambodia, AS996-9, OM4900, OM8108 for Vietnam, Sin Thwe Lat, Sin Thu Kha, Thee Thup Yip for Myanmar, and Ciherang and Inpari 19 for Indonesia to develop high yield potential rice varieties adaptable to target countries for exporting rice seed. Four Indian basmati varieties Basmati 370, DP, Pusa1121 and Sharbati were crossed to diverse genetic diversity of aromatic rice variety. Out of 813 F1 cross combinations, 607 F1s were cultured and evaluated to heading date, plant type, agronomic traits, and grain type, 106 F1s selected and advanced as F2 populations, and 31 F1s selected in 2016 wet season would be advanced in 2017 dry season. 106 F2 populations were evaluated to heading date, plant type, agronomic traits, and grain type, and yield-component traits and selected 2,560 plants in 62 F2 populations. These selected plants were advanced to F3 lines. During six seasons in 2014∼2016, the lines of F3 subsequent generation were cultured a total of 6,256 lines in the range 508∼1,680 lines. These lines were evaluated to heading date, plant type, agronomic traits, grain type and yield-component traits and selected to in three plants in each promising lines. Under climate change, we crossed with diverse germplasm to enhance the resistance to biotic and abiotic stresses. Many lines selected were from HZB-SAL6–SAL3, HHZ8-SAL14-SAL3-Y2, HHZ8-SAL12-Y2-DT1 for salinity, IR79643-39-2-2-3, IR78581-12-3-2-2 for drought, Dular and N22 for heat tolerance, Angke for Xa4 and xa5 to bacterial blight, Taebaeg for Pi5 gene to blast and TW16 for virus. Also, many promising lines developed from the crosses with aromatic rice varieties, Senpidao from Cambodia and Sharbati from India were clear grain of no chalkiness in core and valley. During 2014 and dry season in 2015, five lenes including YR29200-1-1-12-2 from the cross between a Tongil-type Milyang240 and IR78581-12-3-2-2 were selected as promising lines of long grain adaptable to tropical environment. These lines would be used as materials for increasing yield potential and widening genetic diversity in GSP project. In observational yield trial (OYT) for 33 elite lines, two lines of KR49-1-1 and KR55-30-2 were early flowering group of 97 days, 25 lines of KR55-30-2, and so on were medium flowering group of 104∼110 days, and five lines were mid-late flowering group of 112∼114 days in growth duration. The weight of 1000-brown rice grains were 17.2∼21.1g, and the length of brown rice were 6.38∼7.01 mm. The yield of rough rice were an average of 4.9 ton/ha in the range of 3.47 ∼6.02. Nine lines of KR52-10-1, KR52-18-1, KR55-40-1, KR57-3-2, KR57-3-3, KR62-49-1, KR66-9-3, KR76-14-1, KR106-48-5 were 5.42∼6.02 MT/ha of higher 4∼16% than that of Chulsa in rough rice yield. Especially, the yield of five lines KR55-40-1, KR57-3-3, KR66-9-3, KR76-14-1, KR106-48-5 were similar or higher than that of IR66. In replicated yield trial (RYT), the growth duration from sowing to harvesting was 106∼114 days. Five lines including KR530-47-3-2-1-1 were medium flowering group of 106∼110 days, and seven lines including KR57-5-2 were mid-late flowering group. These lines were 76∼99 in number of grain per panicle, an average of 84.6% in the range of 79.3∼91.9% in the percentage of ripened grain and 21.7∼26.5g of the weight of 1000-rough rice grain. The rough rice yield was an average of 5.23 ton/ha in the range of 4.33∼6.06 ton/ha. The yield was increased to 5∼47% than Chulsa and 12∼41% than IR66. Five lines, KR54-28-1, KR55-14-2, KR57-5-2, KR67-57-2 and KR128-19-1 of 5.33∼6.06 ton/ha in the yield were developed from the crosses among the varieties of targeting countries for seed export. These lines were promised as clear appearance and no chalkiness of long grain type over 6.5mm. The promised lines in OYT and RYT trials, would be evaluating to the adaptability and yield potential trials in Vietnam, Myanmar in 2017 dry season. In Cuulong Delta Rice Research Institute (CLRRI) of Mekong Delta region, ten Tongil-type and heat tolerance lines were evaluated to adaptability and yield potential. The growth durations were 104~109 days similar to two Mekong Delta region leading varieties OM7347 and OM6162 of 106 and 108 days. These lines were 13~20 of the number of panicle per hill, 104~162 of the number of grain per panicle, an average of 81.8% in the range of 70.6~88.3% of ripened grain percentage and 21.9~27.9g in the weight of 1000-rough rice grain. Nine lines except for YR29200-37-3-2 were 7.2∼8.6 ton/ha of rough rice yield. However, because they showed not clearance and some chalkiness, there must be improved to grain quality and grain type. In Hlegu region of Yangon Region in Myanmar, eleven Tongil-type and heat tolerance lines including a japonica variety adaptable to tropical region were evaluated to adaptability and yield potential. Most lines were in mid-late group of 100∼108 days of growth duration, 112∼158 in the grain number per panicles, and 85.2∼95.9% in ripened grain ratio, respectively. The yields were an average of 4.35 ton/ha in the range of 3∼5.75 ton/ha. The yield potentials of WC625-1-1-3-1-1-1, KR689-B- 5-1-3 and KR528-b-b-95-2 were 80~92% to IR66, and others were 48~76%. The medium bold grain types of Tongil-type and heat tolerance showed low yield potential in Myanmar. The resistance to blast, bacterial blight and brown planthopper were evaluated in hot-spot regions in southern and northern Vietnam. In the blast nursery of southern hot-spot, twelve varieties/lines were medium resistance and others were resistance except for KR49-1-1 of susceptible. In the blast nursery of northern hot-spot, six core germplasm of Taebaeg, IR78581-12-3-2-2, Angke, Conde, OM8108, and Sin Thu Kha and 16 lines showed moderately resistance and/or resistance. Eighteen lines/varieties including five core germplasm of IR78581-12-3-2-2, OM8108, Angke (Xa4, xa5), Conde (Xa4, Xa7), Sin Thu Kha showed resistance in the evaluation of bacterial blight in northern hot-spot. In virus symptom evaluation, 44 lines/varieties including Taebaeg, IR78581-12-3-2-2 and OM8108 showed to symptom of tungro, yellowing, ragged stunt and grassy stunt. In future, we would try to enhance the resistance using target core resistance genes, Pi5 and Pi40 to blast, xa5 and Xa21 to bacterial blight, Bph1 and Bph40 to brown planthopper and a germplasm TW16 to virus. Sub-project 2: Development of early mature medium short grain rice adaptable to mediterranean countries During the project we evaluated and collected 194 rice germplasms that bred in Europian countries including Turkey and Uzbekistan for developing elite rice cultivars adaptable in Mediterranean and Europian countries. Among them, 154 germplasms were cataloged and stored in Nation agricultural germplasm stock center, Rural Development Administration. We constructed rice breeding base in Braila Agricultural Station in Romania for developing new rice cultivars adaptable to Mediterranean and Europian countries. And also we concluded Memorandum of Agreement with Turkey Agricultural Research Institute and Uzbekistan Scientific Production Center for Agriculture for research collaboration and regional adaptability test of Korean rice cultivars. We evaluated major agronomic traits and field performances of 23 Korean rice cultivars in Romania, Turkey and Uzbekistan from 2014 to 2016. As results, it was found that the most adaptable cultivar in Romanian condition was “Dunae” and “Unkwang” in Turkey. We promoting “Dunae” as a new rice cultivar in Romania and applied to Official Catalog in 2017. For developing new elite rice cultivars, we made 246 genetic crosses from Korean and Europian rice germplasms and bred 3,299 elite rice lines of F1~F4andA1generation from 190 cross combination either. Among rice lines, we could select 9 elite lines finally for preliminary yield test in 2017. We also collected information and analyzed data from rice market, Consuming pattern, breeding goal of Mediterranean and Europian countries. Sub-project 3: Development of molecular markers and bio-assay system of biotic and abiotic stresses for exporting rice Five sets of populations include 211 lines from crosses among high yielding lines including“Boramchan”(Set #1), 53 accessions for crossing(Set #2), 49 selected elite lines(Set #3), 58 advanced breeding and elite lines(Set #4) and 117 advanced and elite lines(Set #5) derived from crosses using heat-tolerant lines“N22” and “Dular”. Lines in Set #2, 3, and #4 were evaluated at 2 sites for major diseases and insect pests. The 53 lines (Set #2) ranged 0 – 9 with a mean of 1.4 for rice blast and averaged 3.2% (ranging 0 –16.2%) for rice ragged stunt virus. Most of the lines were susceptible to BPH except a few lines including Lemont with moderate susceptibility. Six lines including PR34110-B-3-1, CPR34130-B-3-1, IR66, IRRI145, and IR77542-551-1-1-1-1 performed well and are suitable for crossing parents for major diseases. However, no line was resistant to brown planthopper. From screening lines in Set #1, Xa5, Xa1 and Xa21 could be selected for BLB using DNA markers, and for blast resistance Pi2, Pi-39(t), Pi5, and Pi9 genes could be selected using DNA markers. Also, DNA markers showing polymorphisms between parents and linked to Bph20 and Bph21 genes were applicable for marker-aided selection. 2. From lines in Set #2, lines sharing the same bands produced by RM17509 on chromosome 4(Pi63), by RM7213 on chromosome 6 (Pi-zt) and RM27933 on chromosome 12 (Pita) showed resistance scores for rice blast suggesting that a combination of three resistance genes would be effective for rice blast at two test sites. Employment of the Xa1 gene could be effective in enhancing the resistance level against BLB whereas Bph3 on chromosome 6 and Bph17 on chromosome 4 would be useful in reducing the damage by brown planthopper. Lines in Set #4, were screened for diseases and insect pests in dry and wet seasons in 2016. Based on the phenotypic performances and the reactions to diseases and insect pests, some lines were selected as crossing parents and promising lines for further evaluation. Tetep and Tequing were promising as crossing parents from Set #2 and six lines including Milyang23 and YR29200-37-3-2 were selected as crossing parents from Set #3. YR29200-1-1-14-3, KR57-3-2 and KR60-17-1 performed better than the other lines in Set #4 and these promising lines will be tested for additional screening as possible candidates for rice export. The markers closely associated with resistance to these major diseases and insect pets can be used to select for lines with enhanced resistance in the breeding program for rice export. |